ABSTRACT
Objective: To investigate the chemical constituents from the ethanol extract of aerial parts of Clematis manshurica. Methods: The compounds were isolated and purified by chromatography on silica gel, ODS, and preparative HPLC. Their structures were elucidated on the basis of chemical and spectroscopic methods, including MS, 1D, and 2D NMR spectral techniques. Results: Seventeen compounds were isolated from the ethanol extract of the aerial parts of C. manshurica, and were identified as (+)-epipinoresinol (1), (+)-pinoresinol (2), (-)-episyringaresinol (3), (+)-medioresinol (4), (+)-pinoresinol-4-O-β-D-glucopyranoside (5), (+)-syringaresinol-4-O-β-D-glucopyranoside (6), (+)-episyringaresinol-4-O-β-D-glucopyranoside (7), matairesinol (8), (+)-lyoniresinol (9), (+)-isolariciresinol (10), (7R, 8R)-4, 7, 9, 9'-tetrahydroxy-3, 3'-diethoxy-8-O-4'-neolignan (11), (7R, 8S)-4, 7, 9, 9'-tetrahydroxy-3, 3'-diethoxy-8-O-4'-neolignan (12), (7S, 8R)-dihydrodehydroconiferyl alcohol (13), luteolin (14), 3″-O-(2‴- methylbutyryl) isoswertisin (15), 2″-O-(2‴-methylbutyryl) isoswertisin (16), and 6″-O-(2‴-methylbutyryl) isoswertisin (17). Conclusion: All compounds are obtained from the aerial parts of this plant for the first time, and compounds 1, 3, 4, 7-9, 11, 12, and 15-17 are firstly isolated from the plants of Clematis L.